For My Design, I relied heavily on the workflow and code of Dominika Wawrzyniak. Her design was a stylized map of the earth. I used imageJ to convert the Genspace logo into a css file of circle coordinates and sizes. I saved it to GitHub and then used her code with my CSV file. As my design has different size dots, I added a few lines of code to be able to pipette different sizes.
Post Lab:
Write a description about what you intend to do with automation tools for your final project. You may include example pseudocode or Python scripts, procedures you may need to automate, 3D printed holders you may need, and more.
Find and describe a published paper that utilizes the Opentrons or similar automation tools to achieve novel biological applications (eg automated PACE
My plan is to make a genetic circuit of some sort or another. I have been thinking that I would like to use the opentron to perform the transformations that I will need to do.
https://www.sciencedirect.com/science/article/pii/S2472630324000876
Automation of biochemical assays using an open-sourced, inexpensive robotic liquid handler
This paper describes a protocol where the opentron was used to perform a Picogreen assay and a Bradford assay in order to compare its performance as compared to performing the assays by hand as well as by a Tecan Evo liquid handling robot. These two assays are a DNA assay and a protein assay, two assays that are used very often in vaccine development. They found that the robot was very accurate and precise. It was much faster than by hand, but slightly slower than the Tecan Evo. This was due to its lack of 96-channel pipetting head that is used to produce bulk transfers. They liked the small size and cost, however they found that the robot had various limitations compared to the alternative, and that it required a lot more supervision.