What I want to discuss with Dominika:
Where i could get Yarrowia lipolytica?
Exploring in -vitro/in-vivo methods
crisper screens
microfluidic high throughput screening
what could the list of experiments be that I would need to perform?
Who would be the best person to mentor me?
What is the objective? Do I want it to produce more
One or two concrete that I want to explore.
make it higher quality
turning off
How are you going to collect it?
one or two genes in the pathway.
A good story!!!!!!!
https://www.fishersci.com/shop/products/microbiologics-i-yarrowia-lipolytica-i-atcc-9773-3/p-4272654 $211 for 2 pellets
Oleaginous yeast Yarrowia lipolytica IFP29 (ATCC 20460) was grown during 16h in a shake flask containing 100 mL of YEPD Broth using a shaking incubator at 200 rpm, 28 °C. The fermentation was then carried out in a 3.6 L fermenter (Labfors 4, Infors-HT) stirred with two Rushton Impellers. The oleaginous microorganism was grown in batch mode in 1 L of an industrial substrate containing hydrolyzed starch diluted to an initial concentration in glucose of 60 g/L at 28°C and adjusted to pH 4.5. After consumption of the glucose, the fed-batch mode was started with a solution of dextrose syrup (Sirodex, Tereos Syral) and a solution of NH4OH as nitrogen source. The industrial substrate (by-product from a starch plant) was obtained from Tereos Syral. The hydrolysis of starch to glucose was carried out during 24h at 55°C and pH 4.5 using a commercial amyloglucosidase (Spirizyme Fuel HS, Novozymes) at a concentration of 120 µl/L. After 7 hours of fermentation, the biomass was harvested by centrifugation at 5000 rpm for 15 min and washed once with demineralized water to remove the residual nutrients. All the fermentation musts used for the extractions contains 9.93 % of dry matter.
we have this!
Materials and Methods